Real time PCR was performed with 1 Ојl of cDNA and a master mix containing 10 Ојl of Taq Man 2 PCR Master Mix Applied Biosystems, Branchburg, NJ, 8 Ојl nuclease free water, and 1 Ојl of Taq Man probe, and primer sets for the H ATPase B1 subunit using the Applied Biosystems 7500 Fast Real Time PCR System Applied Biosystems, Foster City, CA <a href=
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